UBL5 Modulates Expression of Crucial TLR Pathway Genes and Inflammasome Activation in Human Macrophage Cells

Expression of Endoderm and Hepatic Specific Genes after in vitro Differentiation of Human Embryonic Stem Cells

Background: Human embryonic stem cells (hESC), which are derived from the inner cell mass of the blastocysts, have been considered to be pluripotent cells. In this study we examine the differentiating potential of hESC into hepatocytes by characterization of the expression of endoderm and liver-specific genes. Methods: hESC were cultivated in suspension to form aggregates, the embryoid bodies. ...

Impact of human monocyte and macrophage polarization on NLR expression and NLRP3 inflammasome activation

Inflammasomes are multiprotein complexes nucleating around an NLR (Nucleotide-binding domain and Leucine-rich Repeat containing protein), which regulate the secretion of the pro-inflammatory interleukin (IL)-1β and IL-18 cytokines. Monocytes and macrophages, the main cells expressing the inflammasome genes, adapt to their surrounding microenvironment by a phenotypic polarization towards a pro-i...

Hemin Induces the Activation of NLRP3 Inflammasome in N9 Microglial Cells

Background: Hemin is an important sterile component that induces a neuroinflammatory response after intracerebral hemorrhage, in which NLRP3 inflammasome activation has also proved to be involved. Although microglial activation acts as a key contributor in the neuroinflammatory response, the relationship between hemin and NLRP3 in microglia remains poorly understood. Objective: To investigate w...

Pathway Activation in Immune Cells Radiation Exposure Induces Inflammasome

P-33: Expression of Toll-Like Receptor 2-3 Genes in Human Sertoli Cells

Background: Toll-like receptors (TLRs) constitute a major part of innate immunity, which can distinguish pathogen associate molecular pattern. Sertoli cells create a special immunological niche that protects somniferous tubules from auto antigens and pathogens. These cells are the only somatic cells in somniferous that protect testis cells against pathogens. The purpose of this study was to eva...